FastQC Summary

Read Totals

Library Sizes ranged between 31,935,329 and 38,749,691 reads.

Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.

FastQC Summary

Summary of FastQC flags for each parameter

Per Base Sequence Quality

Heatmap showing mean base qualities for each library

Per Sequence Quality Scores

Heatmap showing mean sequence qualities for each library

Per Base Sequence Content

Heatmap of summed base distributions along each read

Per Sequence GC Content

GC Content Heatmap normalised to theoretical GC content in the Drerio Transcriptome

GC Content Distributions for all reads showing theoretical GC content from the Drerio Transcriptome

Sequence Length Distribution

Sequence Duplication Levels

Adapter Content

Universal Adapter Content

Overrepresented Summary

Total overrepresented sequences for each library

Overrepresented Sequences

## Overrepresented_sequences missing from SRR11951228Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951229Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951230Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951231Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951232Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951233Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951234Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951235Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951236Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951237Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951238Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951239Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951240Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951241Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951242Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951243Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951244Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951245Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951246Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951247Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951248Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951249Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951250Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from SRR11951251Aligned.sortedByCoord.out.bam
## No overrepresented sequences were detected by FastQC

Session Information

R version 4.1.0 (2021-05-18)

Platform: x86_64-pc-linux-gnu (64-bit)

locale: LC_CTYPE=en_AU.UTF-8, LC_NUMERIC=C, LC_TIME=en_AU.UTF-8, LC_COLLATE=en_AU.UTF-8, LC_MONETARY=en_AU.UTF-8, LC_MESSAGES=en_AU.UTF-8, LC_PAPER=en_AU.UTF-8, LC_NAME=C, LC_ADDRESS=C, LC_TELEPHONE=C, LC_MEASUREMENT=en_AU.UTF-8 and LC_IDENTIFICATION=C

attached base packages: parallel, stats, graphics, grDevices, utils, datasets, methods and base

other attached packages: pander(v.0.6.4), DT(v.0.18), scales(v.1.1.1), readr(v.1.4.0), dplyr(v.1.0.7), stringr(v.1.4.0), magrittr(v.2.0.1), ngsReports(v.1.8.1), tibble(v.3.1.2), ggplot2(v.3.3.5) and BiocGenerics(v.0.38.0)

loaded via a namespace (and not attached): Rcpp(v.1.0.7), lubridate(v.1.7.10), here(v.1.0.1), lattice(v.0.20-44), tidyr(v.1.1.3), Biostrings(v.2.60.1), zoo(v.1.8-9), assertthat(v.0.2.1), rprojroot(v.2.0.2), digest(v.0.6.27), utf8(v.1.2.1), plyr(v.1.8.6), R6(v.2.5.0), GenomeInfoDb(v.1.28.1), stats4(v.4.1.0), evaluate(v.0.14), httr(v.1.4.2), pillar(v.1.6.1), zlibbioc(v.1.38.0), rlang(v.0.4.11), lazyeval(v.0.2.2), rstudioapi(v.0.13), data.table(v.1.14.0), jquerylib(v.0.1.4), S4Vectors(v.0.30.0), rmarkdown(v.2.9), labeling(v.0.4.2), htmlwidgets(v.1.5.3), RCurl(v.1.98-1.3), munsell(v.0.5.0), compiler(v.4.1.0), xfun(v.0.24), pkgconfig(v.2.0.3), htmltools(v.0.5.1.1), tidyselect(v.1.1.1), GenomeInfoDbData(v.1.2.6), IRanges(v.2.26.0), fansi(v.0.5.0), viridisLite(v.0.4.0), crayon(v.1.4.1), withr(v.2.4.2), MASS(v.7.3-54), bitops(v.1.0-7), grid(v.4.1.0), jsonlite(v.1.7.2), gtable(v.0.3.0), lifecycle(v.1.0.0), DBI(v.1.1.1), stringi(v.1.6.2), farver(v.2.1.0), reshape2(v.1.4.4), XVector(v.0.32.0), bslib(v.0.2.5.1), ellipsis(v.0.3.2), ggdendro(v.0.1.22), generics(v.0.1.0), vctrs(v.0.3.8), RColorBrewer(v.1.1-2), tools(v.4.1.0), forcats(v.0.5.1), glue(v.1.4.2), purrr(v.0.3.4), crosstalk(v.1.1.1), hms(v.1.1.0), yaml(v.2.2.1), colorspace(v.2.0-2), BiocManager(v.1.30.16), plotly(v.4.9.4.1), knitr(v.1.33) and sass(v.0.4.0)